Views: 88 Author: Site Editor Publish Time: 2023-02-20 Origin: Site
The analytical methods for Rosemary Extract include High-Performance Liquid Chromatography (HPLC), Micellar Electrokinetic Chromatography (MEC), Ultraviolet Spectroscopy (UV), Infrared Spectroscopy (IR), Capillary Electrophoresis (CE), Gas Chromatography (GC), Mass Spectrometry (MS), Nuclear Magnetic Resonance Spectrometry (NMR), etc. Let's have a look at the analytical methods for Rosemary Extract.
The following is a list of contents:
High-Performance Liquid Chromatography (HPLC)
Other methods
The detector is a photodiode array detector (DAD) that allows all wavelengths of light to pass through the flow cell, and then through a series of spectroscopic techniques, all wavelengths of light are detected on the acceptor, using deuterium and tungsten lamps as light sources, covering the spectral region of 190~800nm, which can effectively limit the baseline noise and improve the sensitivity at higher wavelengths. It is a new type of UV detector that can obtain time, intensity, and wavelength simultaneously.
An evaporative light scattering detector (ELSD) is a new general-purpose detector that can detect organic components without UV absorption or with absorption only at the UV end. With the continuous improvement and refinement of the technology, LEDs are increasingly used in pharmaceutical, chemical, and food applications. Compared with other detectors, it eliminates solvent interference and baseline drift caused by temperature changes, and is especially suitable for gradient elution. ELSD can also be used with MS general chromatographic conditions, complementing each other, and plays an important role in the highly sensitive, structural analysis of substances.
HPLC is mainly applied to the quantitative (external standard method) analysis of Rosemary Extract. The parameters measured include reproducibility, recovery, stability, and relative standard error of precision (RSD) to determine the reproducibility, reliability stability, and precision of the analytical results, as well as to evaluate whether the linear correlation (R2) of the external standard curve is within a reasonable interval. According to the existing research reports, it is usually considered that when the RSD of the measured experimental results is less than 1%, it indicates good reproducibility, and when the RSD of the recovery is less than 2%, it is more reliable, when the RSD of the measured peak area is less than 3%, it indicates good stability, and when the RSD of the measurement precision is less than 3.5%, it indicates good precision. The range of the standard line of the external standard method is based on the actual content range of Rosemary Extract to be detected, and then the accuracy of the standard curve is determined by examining the linearity correlation.
Other methods for the determination of Rosemary Extract include 1HNMR, 13CNMR, IR, MS, GC, CE, and the combination of several assays, each with its analytical characteristics.
1. Gas chromatography uses the difference in the distribution coefficients of different substances in the mobile phase and stationary phase so that different compounds flow out of the column at different times to achieve the purpose of high separation.
2. Mass spectrometry uses the law of motion of charged particles in a magnetic or electric field to achieve separation and analysis according to their mass-to-charge ratio (m/z) and to determine the mass of Rosemary Extract ions and their intensity distribution.
3. The NMR method can specifically analyze the structure of organic substances containing hydrogen atoms, penetrate the internal surface of the substance without destroying the sample, and has the advantage of rapid and precise resolution.
4. UV-Vis spectrophotometry is mainly used for the quantitative analysis of Rosemary Extract, but it can also be used for purity tests, preliminary structural speculation of compounds, and comparison with standards and standard spectra to determine the presence of substances.
5. The combination of methods allows the advantages and disadvantages of several instruments to complement each other, providing greater analytical advantages. These methods are mainly used for the qualitative and quantitative analysis of Rosemary Extract.
The above is the relevant content about Rosemary Extract analysis and determination methods, if you are interested in Rosemary Extract, you can contact us, our website is www.gzjahwachem.com.
